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astrocyte specific promoter gfap sequence  (Addgene inc)


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    Addgene inc astrocyte specific promoter gfap sequence
    Fig. 4 The expression of C2 fusion proteins in ex vivo organotypic hippocampal slices after AAV delivery. a OHSC were transduced with AAV carrying either C2-SNAP or C2m2-SNAP at 5 days in vitro (5DIV) and maintained for 9 days for protein expression. The secretion of recombinant proteins was inhibited by 10 µg/ml brefeldin A (BFA) for 5 h. Fixed control (CTRL) and inhibited (BFA) slices were labelled by immunohistochemistry and SNAP-tag substrate for fluorescent imaging. Created by Biorender.com. b Confocal images of OHSC transduced with either AAV-C2-SNAP or AAV-C2m2-SNAP. Cell nuclei were stained with DAPI, fused SNAP-tag was labelled with AlexaFluor647 (AF647). Astrocytes, microglia and neurons were labelled with <t>GFAP,</t> Iba1 antibodies, or expressed EGFP, respectively. Scale bar 20 µm. c, d Quantification of C2-SNAP and C2m2-SNAP fluorescence within astrocytes with or without BFA treatment. Data presented as means ± standard error of the mean (n = 10–15 images per replicate, two independent replicates). Means were compared by one-way ANOVA and post-hoc Tukey test. p values < 0.05 were considered as significant. RFU relative fluorescence units
    Astrocyte Specific Promoter Gfap Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/astrocyte specific promoter gfap sequence/product/Addgene inc
    Average 93 stars, based on 5 article reviews
    astrocyte specific promoter gfap sequence - by Bioz Stars, 2026-06
    93/100 stars

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    1) Product Images from "Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling."

    Article Title: Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling.

    Journal: Cellular & molecular biology letters

    doi: 10.1186/s11658-023-00472-7

    Fig. 4 The expression of C2 fusion proteins in ex vivo organotypic hippocampal slices after AAV delivery. a OHSC were transduced with AAV carrying either C2-SNAP or C2m2-SNAP at 5 days in vitro (5DIV) and maintained for 9 days for protein expression. The secretion of recombinant proteins was inhibited by 10 µg/ml brefeldin A (BFA) for 5 h. Fixed control (CTRL) and inhibited (BFA) slices were labelled by immunohistochemistry and SNAP-tag substrate for fluorescent imaging. Created by Biorender.com. b Confocal images of OHSC transduced with either AAV-C2-SNAP or AAV-C2m2-SNAP. Cell nuclei were stained with DAPI, fused SNAP-tag was labelled with AlexaFluor647 (AF647). Astrocytes, microglia and neurons were labelled with GFAP, Iba1 antibodies, or expressed EGFP, respectively. Scale bar 20 µm. c, d Quantification of C2-SNAP and C2m2-SNAP fluorescence within astrocytes with or without BFA treatment. Data presented as means ± standard error of the mean (n = 10–15 images per replicate, two independent replicates). Means were compared by one-way ANOVA and post-hoc Tukey test. p values < 0.05 were considered as significant. RFU relative fluorescence units
    Figure Legend Snippet: Fig. 4 The expression of C2 fusion proteins in ex vivo organotypic hippocampal slices after AAV delivery. a OHSC were transduced with AAV carrying either C2-SNAP or C2m2-SNAP at 5 days in vitro (5DIV) and maintained for 9 days for protein expression. The secretion of recombinant proteins was inhibited by 10 µg/ml brefeldin A (BFA) for 5 h. Fixed control (CTRL) and inhibited (BFA) slices were labelled by immunohistochemistry and SNAP-tag substrate for fluorescent imaging. Created by Biorender.com. b Confocal images of OHSC transduced with either AAV-C2-SNAP or AAV-C2m2-SNAP. Cell nuclei were stained with DAPI, fused SNAP-tag was labelled with AlexaFluor647 (AF647). Astrocytes, microglia and neurons were labelled with GFAP, Iba1 antibodies, or expressed EGFP, respectively. Scale bar 20 µm. c, d Quantification of C2-SNAP and C2m2-SNAP fluorescence within astrocytes with or without BFA treatment. Data presented as means ± standard error of the mean (n = 10–15 images per replicate, two independent replicates). Means were compared by one-way ANOVA and post-hoc Tukey test. p values < 0.05 were considered as significant. RFU relative fluorescence units

    Techniques Used: Expressing, Ex Vivo, Transduction, In Vitro, Recombinant, Control, Immunohistochemistry, Imaging, Staining, Fluorescence



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    astrocyte specific promoter gfap sequence  (Addgene inc)
    93
    Addgene inc astrocyte specific promoter gfap sequence
    Fig. 4 The expression of C2 fusion proteins in ex vivo organotypic hippocampal slices after AAV delivery. a OHSC were transduced with AAV carrying either C2-SNAP or C2m2-SNAP at 5 days in vitro (5DIV) and maintained for 9 days for protein expression. The secretion of recombinant proteins was inhibited by 10 µg/ml brefeldin A (BFA) for 5 h. Fixed control (CTRL) and inhibited (BFA) slices were labelled by immunohistochemistry and SNAP-tag substrate for fluorescent imaging. Created by Biorender.com. b Confocal images of OHSC transduced with either AAV-C2-SNAP or AAV-C2m2-SNAP. Cell nuclei were stained with DAPI, fused SNAP-tag was labelled with AlexaFluor647 (AF647). Astrocytes, microglia and neurons were labelled with <t>GFAP,</t> Iba1 antibodies, or expressed EGFP, respectively. Scale bar 20 µm. c, d Quantification of C2-SNAP and C2m2-SNAP fluorescence within astrocytes with or without BFA treatment. Data presented as means ± standard error of the mean (n = 10–15 images per replicate, two independent replicates). Means were compared by one-way ANOVA and post-hoc Tukey test. p values < 0.05 were considered as significant. RFU relative fluorescence units
    Astrocyte Specific Promoter Gfap Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/astrocyte specific promoter gfap sequence/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    astrocyte specific promoter gfap sequence - by Bioz Stars, 2026-06
    93/100 stars
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    Fig. 4 The expression of C2 fusion proteins in ex vivo organotypic hippocampal slices after AAV delivery. a OHSC were transduced with AAV carrying either C2-SNAP or C2m2-SNAP at 5 days in vitro (5DIV) and maintained for 9 days for protein expression. The secretion of recombinant proteins was inhibited by 10 µg/ml brefeldin A (BFA) for 5 h. Fixed control (CTRL) and inhibited (BFA) slices were labelled by immunohistochemistry and SNAP-tag substrate for fluorescent imaging. Created by Biorender.com. b Confocal images of OHSC transduced with either AAV-C2-SNAP or AAV-C2m2-SNAP. Cell nuclei were stained with DAPI, fused SNAP-tag was labelled with AlexaFluor647 (AF647). Astrocytes, microglia and neurons were labelled with GFAP, Iba1 antibodies, or expressed EGFP, respectively. Scale bar 20 µm. c, d Quantification of C2-SNAP and C2m2-SNAP fluorescence within astrocytes with or without BFA treatment. Data presented as means ± standard error of the mean (n = 10–15 images per replicate, two independent replicates). Means were compared by one-way ANOVA and post-hoc Tukey test. p values < 0.05 were considered as significant. RFU relative fluorescence units

    Journal: Cellular & molecular biology letters

    Article Title: Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling.

    doi: 10.1186/s11658-023-00472-7

    Figure Lengend Snippet: Fig. 4 The expression of C2 fusion proteins in ex vivo organotypic hippocampal slices after AAV delivery. a OHSC were transduced with AAV carrying either C2-SNAP or C2m2-SNAP at 5 days in vitro (5DIV) and maintained for 9 days for protein expression. The secretion of recombinant proteins was inhibited by 10 µg/ml brefeldin A (BFA) for 5 h. Fixed control (CTRL) and inhibited (BFA) slices were labelled by immunohistochemistry and SNAP-tag substrate for fluorescent imaging. Created by Biorender.com. b Confocal images of OHSC transduced with either AAV-C2-SNAP or AAV-C2m2-SNAP. Cell nuclei were stained with DAPI, fused SNAP-tag was labelled with AlexaFluor647 (AF647). Astrocytes, microglia and neurons were labelled with GFAP, Iba1 antibodies, or expressed EGFP, respectively. Scale bar 20 µm. c, d Quantification of C2-SNAP and C2m2-SNAP fluorescence within astrocytes with or without BFA treatment. Data presented as means ± standard error of the mean (n = 10–15 images per replicate, two independent replicates). Means were compared by one-way ANOVA and post-hoc Tukey test. p values < 0.05 were considered as significant. RFU relative fluorescence units

    Article Snippet: The resulting pET21aC2m2 plasmid was used to construct pET21a-C2m2-SNAP and pET21a-C2m2-mKate plasmids, following the procedure described above. pAAV plasmid for virus production The plasmids for virus production were designed to carry astrocyte specific promoter GFAP sequence from pAAV-GFAP-mKate plasmid (Addgene plasmid #99129, RRID:Addgene_99129).

    Techniques: Expressing, Ex Vivo, Transduction, In Vitro, Recombinant, Control, Immunohistochemistry, Imaging, Staining, Fluorescence